human gallbladder epithelial cell line gbec  (iCell Bioscience Inc)

Journal: Experimental and Therapeutic Medicine

Article Title: Effect of nicotine on cholesterol gallstone formation in C57BL/6J mice fed on a lithogenic diet

doi: 10.3892/etm.2023.11783

Figure Lengend Snippet: Effect of nicotine on cholesterol metabolism by regulating in the gallbladder epithelial tissues. (A) mRNA levels of bile acid metabolism in the gallbladder epithelial tissues of mice injected with nicotine was measured by quantitative polymerase chain reaction (n=3). (B) Representative western blot analyses and (C-E) immunohistochemical staining of FXR and megalin (magnification, x100). Data are expressed as the mean ± standard deviation of the mean (n=10 mice per group). * P<0.05 vs. the ND group; # P<0.05 vs. the LD group. ND, normal diet; LD, lithogenic diet; H, 6.6 mg/kg/2 days nicotine; L, 1.1 mg/kg/2 days nicotine; NPC1L1, NPC1 like intracellular cholesterol transporter 1; ABC, ATP-binding cassette; SR-BI, scavenger receptor class B member 1; FXR, farnesoid X receptor.

Article Snippet: The human gallbladder epithelial cell line GBEC were purchased from iCell Bioscience Inc. and were cultured in low-glucose DMEM supplemented with 2 mM glutamine, 1% MEM non-essential amino acids solution, 1% MEM vitamin solution, 7.5% FBS and P/S (all from Gibco; Thermo Fisher Scientific, Inc.).

Techniques: Injection, Real-time Polymerase Chain Reaction, Western Blot, Immunohistochemical staining, Staining, Standard Deviation, Binding Assay

Journal: Experimental and Therapeutic Medicine

Article Title: Effect of nicotine on cholesterol gallstone formation in C57BL/6J mice fed on a lithogenic diet

doi: 10.3892/etm.2023.11783

Figure Lengend Snippet: Effect of nicotine on the regulation of FXR/megalin/cubilin in the gallbladder epithelial cells. (A) GBEC cells were treated with 2 µM nicotine for 48 h, then total cell lysates (40 µg) from GBECs were subjected to western blot analysis. (B) Immunofluorescence for megalin in confluent GBECs treated with nicotine for 48 h in the presence of FXR agonist CDCA (10 µM; magnification, x100). * P<0.05 vs. control; # P<0.05 vs. the CDCA. FXR, farnesoid X receptor; CDCA, chenodeoxycholic acid.

Article Snippet: The human gallbladder epithelial cell line GBEC were purchased from iCell Bioscience Inc. and were cultured in low-glucose DMEM supplemented with 2 mM glutamine, 1% MEM non-essential amino acids solution, 1% MEM vitamin solution, 7.5% FBS and P/S (all from Gibco; Thermo Fisher Scientific, Inc.).

Techniques: Western Blot, Immunofluorescence, Control